IGFBP3 antibody (AA 135-277)

Catalog No. ABIN7425635

This Mouse Monoclonal antibody specifically detects IGFBP3 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for IGFBP3 antibody (AA 135-277) (ABIN7425635)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D14

Product Details anti-IGFBP3 Antibody

Binding Specificity: AA 135-277

Purpose: Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Specificity: The antibody is a mouse monoclonal antibody raised against IGFBP3. It has been selected for its ability to recognize IGFBP3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Horse, Mouse, Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Insulin Like Growth Factor Binding Protein 3 (IGFBP3) corresdonding to Gly135~Thr277 with N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blottin:g: 0.5-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGFBP3

Background: BP53, IBP3, Growth Hormone-Dependent Binding Protein, Acid Stable Subunit Of The 140 K IGF Complex

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding