CCL2 antibody (AA 24-99)

Catalog No. ABIN7425674

The Mouse Monoclonal anti-CCL2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CCL2 in samples from Human.

Quick Overview for CCL2 antibody (AA 24-99) (ABIN7425674)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 105#

Product Details anti-CCL2 Antibody

Binding Specificity: AA 24-99

Purpose: Monoclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

Specificity: The antibody is a mouse monoclonal antibody raised against MCP1. It has been selected for its ability to recognize MCP1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Monocyte Chemotactic Protein 1 (MCP1) corresdonding to Gln24~Thr99 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1

Background: CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection