Ubiquitin antibody (AA 1-229)

Catalog No. ABIN7428593

This Mouse Monoclonal antibody specifically detects Ubiquitin in WB, IHC, ICC and IP. It exhibits reactivity toward Human and Rat.

Quick Overview for Ubiquitin antibody (AA 1-229) (ABIN7428593)

Target: Ubiquitin (Ub)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: D2

Product Details anti-Ubiquitin Antibody

Binding Specificity: AA 1-229

Purpose: Monoclonal Antibody to Ubiquitin (Ub)

Specificity: The antibody is a mouse monoclonal antibody raised against Ub. It has been selected for its ability to recognize Ub in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Ubiquitin (Ub) corresdonding to Met1~Cys229 with N-terminal His Tag

Isotype: IgG2 kappa

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway