MBP antibody (AA 220-301)

Catalog No. ABIN7425917

The Mouse Monoclonal anti-MBP antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect MBP in samples from Human.

Quick Overview for MBP antibody (AA 220-301) (ABIN7425917)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: A5

Product Details anti-MBP Antibody

Binding Specificity: AA 220-301

Purpose: Monoclonal Antibody to Myelin Basic Protein (MBP)

Specificity: The antibody is a mouse monoclonal antibody raised against MBP. It has been selected for its ability to recognize MBP in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Myelin Basic Protein (MBP) corresdonding to Val220~Met301 with N-terminal His and GST Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein

Background: Myelin A1 protein, Myelin membrane encephalitogenic protein

UniProt: P02686