EGF antibody (AA 974-1026)

Catalog No. ABIN7427390

The Mouse Monoclonal anti-EGF antibody has been validated for WB, IHC, IP, ICC. It is suitable to detect EGF in samples from Rat.

Quick Overview for EGF antibody (AA 974-1026) (ABIN7427390)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C11

Product Details anti-EGF Antibody

Binding Specificity: AA 974-1026

Purpose: Monoclonal Antibody to Epidermal Growth Factor (EGF)

Specificity: The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Epidermal Growth Factor (EGF) corresdonding to Asn974~Arg1026 with N-terminal His and GST Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blottin:g: 0.01-5 μg/mL,Immunohistochemistry: 5-50 μg/mL,Immunocytochemistry: 5-50 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: Epidermal Growth Factor

Background: URG, Beta-Urogastrone, Pro-epidermal growth factor

UniProt: P07522

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation