S100B antibody (AA 1-92)

Catalog No. ABIN7425947

The Mouse Monoclonal anti-S100B antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect S100B in samples from Human.

Quick Overview for S100B antibody (AA 1-92) (ABIN7425947)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D5

Product Details anti-S100B Antibody

Binding Specificity: AA 1-92

Purpose: Monoclonal Antibody to S100 Calcium Binding Protein B (S100B)

Specificity: The antibody is a mouse monoclonal antibody raised against S100B. It has been selected for its ability to recognize S100B in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant S100 Calcium Binding Protein B (S100B) corresdonding to Met1~Glu92 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B

Background: S100-B, NEF, S100Beta, S-100 protein subunit beta

UniProt: P04271

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins