Prothrombin antibody

Catalog No. ABIN7426067

This Mouse Monoclonal antibody specifically detects Prothrombin in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Prothrombin antibody (ABIN7426067)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: E1

Product Details anti-Prothrombin Antibody

Purpose: Monoclonal Antibody to Coagulation Factor II (F2)

Specificity: The antibody is a mouse monoclonal antibody raised against F2. It has been selected for its ability to recognize F2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Coagulation Factor II (F2) corresdonding to N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-4 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Coagulation Factor II

Background: FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling