NOS2 antibody (AA 518-694)

Catalog No. ABIN7427453

This Mouse Monoclonal antibody specifically detects NOS2 in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for NOS2 antibody (AA 518-694) (ABIN7427453)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C3

Product Details anti-NOS2 Antibody

Binding Specificity: AA 518-694

Purpose: Monoclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2)

Specificity: The antibody is a mouse monoclonal antibody raised against NOS2. It has been selected for its ability to recognize NOS2 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Nitric Oxide Synthase 2, Inducible (NOS2) corresdonding to Ala518~Arg694 with N-terminal His Tag

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunofluorescence:5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: NOS2

Background: NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2

UniProt: Q06518

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process