Clusterin antibody (AA 229-446)

Catalog No. ABIN7427705

The Mouse Monoclonal anti-Clusterin antibody has been validated for WB and IHC. It is suitable to detect Clusterin in samples from Pig.

Quick Overview for Clusterin antibody (AA 229-446) (ABIN7427705)

Target: Clusterin (CLU)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: D18

Product Details anti-Clusterin Antibody

Binding Specificity: AA 229-446

Purpose: Monoclonal Antibody to Clusterin (CLU)

Specificity: The antibody is a mouse monoclonal antibody raised against CLU. It has been selected for its ability to recognize CLU in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Clusterin (CLU) corresdonding to Ile229~Glu446 (Accession # Q29549)

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background: CLU, TRPM2, KUB1, CLI, APOJ, SGP2, AAG4, APO-J, SP-40, Apolipoprotein J, Complement Lysis Inhibitor, 40,Sulfated Glycoprotein 2, Testosterone-Repressed Prostate Message 2

UniProt: Q29549

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling