HFE2 antibody (AA 29-401)

Catalog No. ABIN7428074

The Mouse Monoclonal anti-HFE2 antibody is suitable to detect HFE2 in samples from Human. It has been validated for WB.

Quick Overview for HFE2 antibody (AA 29-401) (ABIN7428074)

Target: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HFE2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A7

Product Details anti-HFE2 Antibody

Binding Specificity: AA 29-401

Purpose: Monoclonal Antibody to Hemojuvelin (HJV)

Specificity: The antibody is a mouse monoclonal antibody raised against HJV. It has been selected for its ability to recognize HJV in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Hemojuvelin (HJV) corresdonding to Leu29~Ala401 (Accession # Q6ZVN8)

Isotype: IgG2b lambda

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HFE2

Target: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Alternative Name: Hemojuvelin

Background: HFE2, HFE2A, HJV, JH, RGMC, hemochromatosis type 2(Juvenile), Haemojuvelin, RGM domain family member C

UniProt: Q6ZVN8

Pathways: Transition Metal Ion Homeostasis