HDAC1 antibody (AA 1-482)

Catalog No. ABIN7426493

This anti-HDAC1 antibody is a Mouse Monoclonal antibody detecting HDAC1 in WB, IHC, IF, ICC and FACS. Suitable for Human.

Quick Overview for HDAC1 antibody (AA 1-482) (ABIN7426493)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: E22

Product Details anti-HDAC1 Antibody

Binding Specificity: AA 1-482

Purpose: Monoclonal Antibody to Histone Deacetylase 1 (HDAC1)

Specificity: The antibody is a mouse monoclonal antibody raised against HDAC1. It has been selected for its ability to recognize HDAC1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Histone Deacetylase 1 (HDAC1) corresdonding to Met1~Ala482 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunofluorescence:5-20 μg/mL,Flow cytometry: 10 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background: HDAC1, GON10, HD1, RPD3, RPD3L1

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis