CD3 epsilon antibody (AA 28-185)

Catalog No. ABIN7428527

This Mouse Monoclonal antibody specifically detects CD3 epsilon in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for CD3 epsilon antibody (AA 28-185) (ABIN7428527)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C10

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 28-185

Purpose: Monoclonal Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e)

Specificity: The antibody is a mouse monoclonal antibody raised against CD3e. It has been selected for its ability to recognize CD3e in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) corresdonding to Met28~Asn185 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: T3E, TCRE, CD3-E Molecule,Epsilon(CD3-TCR Complex), T-cell surface antigen T3/Leu-4 epsilon chain

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway