SFRP1 antibody (AA 32-314)

Catalog No. ABIN7427168

The Mouse Monoclonal anti-SFRP1 antibody has been validated for WB and IHC. It is suitable to detect SFRP1 in samples from Human.

Quick Overview for SFRP1 antibody (AA 32-314) (ABIN7427168)

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C5

Product Details anti-SFRP1 Antibody

Binding Specificity: AA 32-314

Purpose: Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1)

Specificity: The antibody is a mouse monoclonal antibody raised against SFRP1. It has been selected for its ability to recognize SFRP1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Secreted Frizzled Related Protein 1 (SFRP1) corresdonding to Ser32~Lys314 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: SFRP1

Background: FRP1, FRP, FRP-1, FrzA, SARP2, Secreted apoptosis-related protein 2

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation