GFAP antibody (AA 254-432)

Catalog No. ABIN7445402

This Rabbit Polyclonal antibody specifically detects GFAP in WB, IHC, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for GFAP antibody (AA 254-432) (ABIN7445402)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-GFAP Antibody

Binding Specificity: AA 254-432

Purpose: Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)

Specificity: The antibody is a rabbit polyclonal antibody raised against GFAP. It has been selected for its ability to recognize GFAP in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glial Fibrillary Acidic Protein (GFAP) corresdonding to Glu254~Met432 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Intermediate Filament Protein

UniProt: P14136