PKC eta antibody (AA 355-614)

Catalog No. ABIN7431425

This anti-PKC eta antibody is a Rabbit Polyclonal antibody detecting PKC eta in WB, ELISA, ICC, IHC (p) and IHC (fro). Suitable for Human.

Quick Overview for PKC eta antibody (AA 355-614) (ABIN7431425)

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC eta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PKC eta Antibody

Binding Specificity: AA 355-614

Purpose: Polyclonal Antibody to Protein Kinase C Eta (PKCh)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKCh. It has been selected for its ability to recognize PKCh in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase C Eta (PKCh) corresdonding to Phe355~Phe614 (Accession # P24723)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PKC eta

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Alternative Name: PKCh

Background: PRKCH, PRKC-H, PKC-L, PRKCL, NPKC-Eta

UniProt: P24723

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling