PKC eta antibody (AA 355-614)

Catalog No. ABIN7431425

The Rabbit Polyclonal anti-PKC eta antibody (ABIN7431425) specifically detects PKC eta in WB, ELISA, IHC (p), ICC and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for PKC eta antibody (AA 355-614) (ABIN7431425)

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC eta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PKC eta Antibody

Binding Specificity: AA 355-614

Purpose: Polyclonal Antibody to Protein Kinase C Eta (PKCh)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKCh. It has been selected for its ability to recognize PKCh in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase C Eta (PKCh) corresdonding to Phe355~Phe614 (Accession # P24723)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PKC eta

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Alternative Name: PKCh

Background: PRKCH, PRKC-H, PKC-L, PRKCL, NPKC-Eta

UniProt: P24723

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling