Histone H4 antibody (AA 2-103)

Catalog No. ABIN7440314

This independently validated Rabbit Polyclonal antibody specifically detects Histone H4 in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Chicken, Cat, Dog, Guinea Pig, Horse and Rhesus Monkey.

Quick Overview for Histone H4 antibody (AA 2-103) (ABIN7440314)

Target: Histone H4

Reactivity: Human, Mouse, Rat, Pig, Cow, Chicken, Cat, Dog, Guinea Pig, Horse, Rhesus Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Histone H4 Antibody

Binding Specificity: AA 2-103

Purpose: Polyclonal Antibody to Histone H4 (H4)

Specificity: The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Cat, Chicken, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Rhesus Monkey

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Histone H4 (H4) corresdonding to Ser2~Gly103 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #104631 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104631

Date: 08/04/2025

Antigen: Histone H4

Lot Number: A20250716116

Method validated: Immunohistochemistry

Positive Control:

Negative Control:

Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.

Validation Images

Histone H4 immunoreactive cells in the cerebral cortex and hippocampus of an adult CD1 mouse. Antibody dilution: 1:25. Magnification: 63x.

Full Methods

Primary Antibody: ABIN7440314

Secondary Antibody: anti-rabbit Alexa Fluor 488 (Invitrogen A11008, lot # 3112790)

Full Protocol:

• Sample analyzed: adult (9 months old) CD1 mouse brain.
• Paraffin-embedded slices (7µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
• Sections were blocked in ovalbumin 1% 1 hour at room temperature.
• Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6).
• 3x5 minutes washing in 0.01M PBS.
• Sections were incubated with the primary antibody at the following dilutions of 1:25/1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent).
• 3x5 minutes washing in 0.01M PBS.
• Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
• 3x5 minutes washing in 0.01M PBS.
• Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) 1µg/mL, 2 minutes.
• 2x5 minutes washing in 0.01M PBS.
• Specimens were then mounted in Fluoroshield mounting medium (Sigma).
• Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 63x magnification.

Experimental Notes:

Passed. The antibody works in IHC-P at concentrations from 1:25-1:50.

Handling

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Histone H4

Target: Histone H4