PLA2G6 antibody (AA 484-701)

Catalog No. ABIN7431758

This Rabbit Polyclonal antibody specifically detects PLA2G6 in WB, ELISA, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PLA2G6 antibody (AA 484-701) (ABIN7431758)

Target: PLA2G6 (Phospholipase A2, Group VI (Cytosolic, Calcium-Independent) (PLA2G6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PLA2G6 Antibody

Binding Specificity: AA 484-701

Purpose: Polyclonal Antibody to Phospholipase A2, Calcium Independent (iPLA2)

Specificity: The antibody is a rabbit polyclonal antibody raised against iPLA2. It has been selected for its ability to recognize iPLA2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phospholipase A2, Calcium Independent (iPLA2) corresdonding to Arg484~Ile701 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PLA2G6

Target: PLA2G6 (Phospholipase A2, Group VI (Cytosolic, Calcium-Independent) (PLA2G6))

Alternative Name: iPLA2

Background: PLA2G6, CaI-PLA2, GVI, INAD1, PNPLA9, Ca2+ Independent PLA2, 85 kDa Calcium-Independent Phospholipase A2, Phospholipase A2,group VI(Cytosolic,Calcium-Independent)

UniProt: O60733

Pathways: Positive Regulation of Peptide Hormone Secretion