PKC iota antibody (AA 375-596)

Catalog No. ABIN7444109

The Rabbit Polyclonal anti-PKC iota antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PKC iota in samples from Human.

Quick Overview for PKC iota antibody (AA 375-596) (ABIN7444109)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PKC iota Antibody

Binding Specificity: AA 375-596

Purpose: Polyclonal Antibody to Protein Kinase C Iota (PKCi)

Specificity: The antibody is a rabbit polyclonal antibody raised against PKCi. It has been selected for its ability to recognize PKCi in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase C Iota (PKCi) corresdonding to Ile375~Val596 (Accession # P41743)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: PKCi

Background: PKC-I, PRKCI, PRKC-I, NPKC-Iota, Atypical protein kinase C-lambda/iota

UniProt: P41743

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation