IgE antibody (AA 1-421)

Catalog No. ABIN7429514

The Rabbit Polyclonal anti-IgE antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect IgE in samples from Mouse.

Quick Overview for IgE antibody (AA 1-421) (ABIN7429514)

Target: IgE

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IgE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-IgE Antibody

Binding Specificity: AA 1-421

Purpose: Polyclonal Antibody to Immunoglobulin E (IgE)

Specificity: The antibody is a rabbit polyclonal antibody raised against IgE. It has been selected for its ability to recognize IgE in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Immunoglobulin E (IgE) corresdonding to Ser1~Ser421 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IgE

Target: IgE

Alternative Name: Immunoglobulin E

Background: IGHE, Immunoglobulin Heavy Constant Epsilon, Ig epsilon chain C region