F12 antibody (AA 400-615)

Catalog No. ABIN7432373

The Rabbit Polyclonal anti-F12 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect F12 in samples from Human.

Quick Overview for F12 antibody (AA 400-615) (ABIN7432373)

Target: F12 (Coagulation Factor XII (F12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This F12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-F12 Antibody

Binding Specificity: AA 400-615

Purpose: Polyclonal Antibody to Coagulation Factor XII (F12)

Specificity: The antibody is a rabbit polyclonal antibody raised against F12. It has been selected for its ability to recognize F12 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Coagulation Factor XII (F12) corresdonding to Ser400~Ser615 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.24 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for F12

Target: F12 (Coagulation Factor XII (F12))

Alternative Name: Coagulation Factor XII

Background: FXII, HAF, Contact Factor, Hageman Factor

UniProt: P00748

Pathways: Complement System