DKK1 antibody (AA 36-260)

Catalog No. ABIN7432502

The Rabbit Polyclonal anti-DKK1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect DKK1 in samples from Rat.

Quick Overview for DKK1 antibody (AA 36-260) (ABIN7432502)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DKK1 Antibody

Binding Specificity: AA 36-260

Purpose: Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against DKK1. It has been selected for its ability to recognize DKK1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Dickkopf Related Protein 1 (DKK1) corresdonding to Val36~Asn260 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: SK, Dickkopf Homolog 1(Xenopus Laevis)

UniProt: D3Z9J1

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation