PD-1 antibody (AA 39-266)

Catalog No. ABIN7442453

This Rabbit Polyclonal antibody specifically detects PD-1 in WB, IHC, ICC and IP. It exhibits reactivity toward Rat.

Quick Overview for PD-1 antibody (AA 39-266) (ABIN7442453)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PD-1 Antibody

Binding Specificity: AA 39-266

Purpose: Polyclonal Antibody to Programmed Cell Death Protein 1 (PD1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PD1. It has been selected for its ability to recognize PD1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Programmed Cell Death Protein 1 (PD1) corresdonding to Pro39~Ser266 (Accession # D3ZIN8) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: Programmed Cell Death Protein 1

Background: CD279, PDCD1, SLEB2, HPD1P

UniProt: D3ZIN8

Pathways: Cancer Immune Checkpoints