LIPC antibody (AA 347-478)

Catalog No. ABIN7429355

The Rabbit Polyclonal anti-LIPC antibody has been validated for WB, ELISA, ICC, IHC (p) and IHC (fro). It is suitable to detect LIPC in samples from Human.

Quick Overview for LIPC antibody (AA 347-478) (ABIN7429355)

Target: LIPC (Lipase, Hepatic (LIPC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIPC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LIPC Antibody

Binding Specificity: AA 347-478

Purpose: Polyclonal Antibody to Lipase, Hepatic (LIPC)

Specificity: The antibody is a rabbit polyclonal antibody raised against LIPC. It has been selected for its ability to recognize LIPC in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Lipase, Hepatic (LIPC) corresdonding to Ser347~Pro478 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for LIPC

Target: LIPC (Lipase, Hepatic (LIPC))

Alternative Name: LIPC

Background: HL, HTGL, Hepatic Lipase, Hepatic triacylglycerol lipase, Lipase member C

UniProt: P11150

Pathways: Lipid Metabolism