SPARC antibody (AA 18-302)

Catalog No. ABIN7432616

The Rabbit Polyclonal anti-SPARC antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect SPARC in samples from Mouse.

Quick Overview for SPARC antibody (AA 18-302) (ABIN7432616)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-SPARC Antibody

Binding Specificity: AA 18-302

Purpose: Polyclonal Antibody to Osteonectin (ON)

Specificity: The antibody is a rabbit polyclonal antibody raised against ON. It has been selected for its ability to recognize ON in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Osteonectin (ON) corresdonding to Ala18~Ile302 (Accession # P07214)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: Osteonectin

Background: SPARC, BM-40, Secreted Protein,Acidic,Cysteine-Rich, Basement-membrane protein 40, Secreted protein acidic and rich in cysteine

UniProt: P07214

Pathways: Autophagy