ENOS antibody (AA 519-702)

Catalog No. ABIN7432802

The Rabbit Polyclonal anti-ENOS antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect ENOS in samples from Rat.

Quick Overview for ENOS antibody (AA 519-702) (ABIN7432802)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-ENOS Antibody

Binding Specificity: AA 519-702

Purpose: Polyclonal Antibody to Endothelial NOS (eNOS)

Specificity: The antibody is a rabbit polyclonal antibody raised against eNOS. It has been selected for its ability to recognize eNOS in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rabbit

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Endothelial NOS (eNOS) corresdonding to Ala519~Phe702 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: eNOS

Background: NOS3, ECNOS, NOS III, E-NOS, C-NOS, ENOS, CNOS, Constitutive NOS, Nitric Oxide Synthase 3, Endothelial

UniProt: Q62600

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling