PPIA antibody (AA 2-164)

Catalog No. ABIN7432967

This anti-PPIA antibody is a Rabbit Polyclonal antibody detecting PPIA in WB, IHC, ICC and IP. Suitable for Rat.

Quick Overview for PPIA antibody (AA 2-164) (ABIN7432967)

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PPIA Antibody

Binding Specificity: AA 2-164

Purpose: Polyclonal Antibody to Cyclophilin A (CYPA)

Specificity: The antibody is a rabbit polyclonal antibody raised against CYPA. It has been selected for its ability to recognize CYPA in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cyclophilin A (CYPA) corresdonding to Val2~Leu164 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:400-1600,Immunohistochemistry: 5-20 μg/mL,1:40-160,Immunocytochemistry: 5-20 μg/mL,1:40-160,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: CYPA

Background: PPIA, CyP-A, CYPH, Peptidylprolyl Isomerase A, Peptidyl-Prolyl Isomerase A, Peptidyl-prolyl cis-trans isomerase A, Cyclosporin A-binding protein

UniProt: P10111