Intestinal Alkaline Phosphatase antibody (AA 20-333)

Catalog No. ABIN7433141

The Rabbit Polyclonal anti-Intestinal Alkaline Phosphatase antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Intestinal Alkaline Phosphatase in samples from Human.

Quick Overview for Intestinal Alkaline Phosphatase antibody (AA 20-333) (ABIN7433141)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Binding Specificity: AA 20-333

Purpose: Polyclonal Antibody to Alkaline Phosphatase, Intestinal (ALPI)

Specificity: The antibody is a rabbit polyclonal antibody raised against ALPI. It has been selected for its ability to recognize ALPI in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Alkaline Phosphatase, Intestinal (ALPI) corresdonding to Val20~Arg333 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: ALPI

Background: CIP, IAP, Calf Intestinal Alkaline Phosphatase

UniProt: P09923