CSPG5 antibody (AA 139-370)

Catalog No. ABIN7442075

The Rabbit Polyclonal anti-CSPG5 antibody is suitable to detect CSPG5 in samples from Rat. It has been validated for WB, IHC, ICC and IP.

Quick Overview for CSPG5 antibody (AA 139-370) (ABIN7442075)

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSPG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-CSPG5 Antibody

Binding Specificity: AA 139-370

Purpose: Polyclonal Antibody to Chondroitin Sulfate Proteoglycan 5 (CSPG5)

Specificity: The antibody is a rabbit polyclonal antibody raised against CSPG5. It has been selected for its ability to recognize CSPG5 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Chondroitin Sulfate Proteoglycan 5 (CSPG5) corresdonding to Ala139~Arg370 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CSPG5

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Alternative Name: CSPG5

Background: NGC, CALEB, Acidic leucine-rich EGF-like domain-containing brain protein, Neuroglycan C

UniProt: Q9ERQ6

Pathways: Glycosaminoglycan Metabolic Process