SV40 Large T Antigen (AA 131-260) antibody
The Rabbit Polyclonal anti-SV40 Large T Antigen antibody (ABIN7445528) specifically detects SV40 Large T Antigen in IP, WB, ICC and IHC.
The antibody is reactive with Various Species samples.
Catalog No. ABIN7445528
$360.20
Plus shipping costs $50.00
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Quick Overview for SV40 Large T Antigen (AA 131-260) antibody (ABIN7445528)
Target
SV40 Large T Antigen
Reactivity
Various Species
Host
Rabbit
Clonality
Polyclonal
Conjugate
Un-conjugated
Application
Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
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Binding Specificity
- AA 131-260
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Purpose
- Polyclonal Antibody to Large T Antigen (LT)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against LT. It has been selected for its ability to recognize LT in immunohistochemical staining and western blotting.
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- Recombinant Large T Antigen (LT) corresdonding to Lys131~Glu260 with N-terminal His Tag
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Isotype
- IgG
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Application Notes
- Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 24 months
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- SV40 Large T Antigen
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Alternative Name
- Large T Antigen
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Target Type
- Viral Protein
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Background
- LT-AG, Simian virus 40 large T antigen
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UniProt
- P03070
Target
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