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Glucagon antibody (AA 23-180)

This Rabbit Polyclonal antibody specifically detects Glucagon in WB, IHC, IP and ICC. It exhibits reactivity toward Human.
Catalog No. ABIN7429775

Quick Overview for Glucagon antibody (AA 23-180) (ABIN7429775)

Target

See all Glucagon (GCG) Antibodies
Glucagon (GCG)

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Glucagon antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    AA 23-180

    Purpose

    Polyclonal Antibody to Glucagon (GCG)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against GCG. It has been selected for its ability to recognize GCG in immunohistochemical staining and western blotting.

    Cross-Reactivity

    Pig

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Glucagon (GCG) corresdonding to Leu23~Lys180 with N-terminal His Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Glucagon (GCG)

    Alternative Name

    Glucagon

    Background

    GC, GLP1, GLP2, GRPP, Glicentin-Related Polypeptide, Glucagen, Oxyntomodulin, Incretin hormone

    UniProt

    P01275

    Pathways

    Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling
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