Elastin antibody (AA 266-443)

Catalog No. ABIN7433492

The Rabbit Polyclonal anti-Elastin antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Elastin in samples from Mouse.

Quick Overview for Elastin antibody (AA 266-443) (ABIN7433492)

Target: Elastin (ELN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Elastin Antibody

Binding Specificity: AA 266-443

Purpose: Polyclonal Antibody to Elastin (ELN)

Specificity: The antibody is a rabbit polyclonal antibody raised against ELN. It has been selected for its ability to recognize ELN in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Elastin (ELN) corresdonding to Pro266~Gly443 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.95 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: Elastin

Background: WBS, WS, SVAS, Tropoelastin, Supravalvular Aortic Stenosis, Williams-Beuren Syndrome

UniProt: P54320

Pathways: Regulation of Actin Filament Polymerization