ALOX15B antibody (AA 253-490)

Catalog No. ABIN7441982

The Rabbit Polyclonal anti-ALOX15B antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ALOX15B in samples from Human.

Quick Overview for ALOX15B antibody (AA 253-490) (ABIN7441982)

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ALOX15B Antibody

Binding Specificity: AA 253-490

Purpose: Polyclonal Antibody to 15-Lipoxygenase-2 (15-LO-2)

Specificity: The antibody is a rabbit polyclonal antibody raised against 15-LO-2. It has been selected for its ability to recognize 15-LO-2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant 15-Lipoxygenase-2 (15-LO-2) corresdonding to Arg253~Glu490 (Accession # O15296)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ALOX15B

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Alternative Name: 15-LO-2

Background: ALOX15B, LOX15B, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Lipoxygenase 15, Type B, 15-lipoxygenase 2, Linoleate 13-lipoxygenase 15-LOb Arachidonate-15-Lipoxygenase, Type B

UniProt: O15296