Hemoglobin antibody (APC)

Catalog No. ABIN7437242

The Guinea Pig Polyclonal anti-Hemoglobin antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect Hemoglobin in samples from Rabbit.

Quick Overview for Hemoglobin antibody (APC) (ABIN7437242)

Target: Hemoglobin

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Hemoglobin antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Hemoglobin Antibody (APC)

Purpose: Polyclonal Antibody to Hemoglobin (HB)

Specificity: The antibody is a cavia polyclonal antibody raised against HB. It has been selected for its ability to recognize HB in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Native Hemoglobin (HB)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Hemoglobin

Target: Hemoglobin

Background: Hgb, Haemoglobin, Heterotetramer(αβ)2