RASA1 antibody (AA 403-596)

Catalog No. ABIN7443047

This Rabbit Polyclonal antibody specifically detects RASA1 in WB, ELISA, IHC (p), IHC (fro) and ICC. It exhibits reactivity toward Human.

Quick Overview for RASA1 antibody (AA 403-596) (ABIN7443047)

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-RASA1 Antibody

Binding Specificity: AA 403-596

Purpose: Polyclonal Antibody to Ras GTPase Activating Protein 1 (RASA1)

Specificity: The antibody is a rabbit polyclonal antibody raised against RASA1. It has been selected for its ability to recognize RASA1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Ras GTPase Activating Protein 1 (RASA1) corresdonding to Pro403~Leu596 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000Immunohistochemistry: 5-20 μg/mL,1:50-200Immunocytochemistry: 5-20 μg/mL,1:50-200Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for RASA1

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Alternative Name: RASA1

Background: Ras-GAP, RasGAP, CM-AVM, CMAVM, GAP, PKWS, RASA, P120GAP, RAS P21 Protein Activator 1, Capillary Malformation-Arteriovenous Malformation

UniProt: P20936

Pathways: Regulation of Actin Filament Polymerization, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals