PDHB antibody (AA 31-341)

Catalog No. ABIN7434347

This anti-PDHB antibody is a Rabbit Polyclonal antibody detecting PDHB in WB, IHC, IP, ICC and FACS. Suitable for Human.

Quick Overview for PDHB antibody (AA 31-341) (ABIN7434347)

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PDHB Antibody

Binding Specificity: AA 31-341

Purpose: Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDHb. It has been selected for its ability to recognize PDHb in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Pyruvate Dehydrogenase Beta (PDHb) corresdonding to Leu31~Asn341 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: PDHb

Background: PDH-B, PHE1B, PHE1-B, Pyruvate Dehydrogenase(lipoamide)Beta

UniProt: P11177

Pathways: Warburg Effect