GLUT4 antibody (AA 228-292)

Catalog No. ABIN7434539

The Rabbit Polyclonal anti-GLUT4 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect GLUT4 in samples from Human.

Quick Overview for GLUT4 antibody (AA 228-292) (ABIN7434539)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GLUT4 Antibody

Binding Specificity: AA 228-292

Purpose: Polyclonal Antibody to Glucose Transporter 4 (GLUT4)

Specificity: The antibody is a rabbit polyclonal antibody raised against GLUT4. It has been selected for its ability to recognize GLUT4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glucose Transporter 4 (GLUT4) corresdonding to Arg228~Val292 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: GLUT4

Background: SLC2A4, Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect