Phospholipase D antibody (AA 725-1074)

Catalog No. ABIN7434611

This Rabbit Polyclonal antibody specifically detects Phospholipase D in WB, IP, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for Phospholipase D antibody (AA 725-1074) (ABIN7434611)

Target: Phospholipase D (PLD)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Phospholipase D Antibody

Binding Specificity: AA 725-1074

Purpose: Polyclonal Antibody to Phospholipase D (PLD)

Specificity: The antibody is a rabbit polyclonal antibody raised against PLD. It has been selected for its ability to recognize PLD in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phospholipase D (PLD) corresdonding to Thr725~Thr1074 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Phospholipase D

Target: Phospholipase D (PLD)

Alternative Name: Phospholipase D

Background: PLD1, Choline Phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1

UniProt: Q13393

Pathways: Response to Water Deprivation, ER-Nucleus Signaling