SMAD3 antibody (AA 231-425)

Catalog No. ABIN7434683

The Rabbit Polyclonal anti-SMAD3 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect SMAD3 in samples from Human.

Quick Overview for SMAD3 antibody (AA 231-425) (ABIN7434683)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 231-425

Purpose: Polyclonal Antibody to SMAD family member 3 (SMAD3)

Specificity: The antibody is a rabbit polyclonal antibody raised against SMAD3. It has been selected for its ability to recognize SMAD3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SMAD family member 3 (SMAD3) corresdonding to Phe231~Ser425 (Accession # P84022)

Isotype: IgG

Application Details

Application Notes: Western blottin:g: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: JV15-2, MADH3, Mothers Against Decapentaplegic Homolog 3

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy