Col4a4 antibody (AA 1469-1690)

Catalog No. ABIN7444712

The Rabbit Polyclonal anti-Col4a4 antibody (ABIN7444712) specifically detects Col4a4 in WB, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Col4a4 antibody (AA 1469-1690) (ABIN7444712)

Target: Col4a4 (Col4A4) (Collagen, Type IV, alpha 4 (Col4A4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Col4a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Col4a4 Antibody

Binding Specificity: AA 1469-1690

Purpose: Polyclonal Antibody to Collagen Type IV Alpha 4 (COL4a4)

Specificity: The antibody is a rabbit polyclonal antibody raised against COL4a4. It has been selected for its ability to recognize COL4a4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Collagen Type IV Alpha 4 (COL4a4) corresdonding to Val1469~Ser1690 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Col4a4

Target: Col4a4 (Col4A4) (Collagen, Type IV, alpha 4 (Col4A4))

Alternative Name: COL4a4

Background: COL4-A4, CA44, Collagen Alpha-4(IV)chain, Collagen Of Basement Membrane,Alpha-4 Chain

UniProt: P53420