NAE1 antibody (AA 229-534)

Catalog No. ABIN7444856

This anti-NAE1 antibody is a Rabbit Polyclonal antibody detecting NAE1 in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for NAE1 antibody (AA 229-534) (ABIN7444856)

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NAE1 Antibody

Binding Specificity: AA 229-534

Purpose: Polyclonal Antibody to Amyloid Beta Precursor Protein Binding Protein 1 (APPBP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against APPBP1. It has been selected for its ability to recognize APPBP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Amyloid Beta Precursor Protein Binding Protein 1 (APPBP1) corresdonding to Thr229~Leu534 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:160-640,Immunohistochemistry: 5-20 μg/mL,1:16-64,Immunocytochemistry: 5-20 μg/mL,1:16-64,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.32 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NAE1

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Alternative Name: APPBP1

Background: NAE1, HPP1, Ula-1, NEDD8-Activating Enzyme E1 Regulatory Subunit 1, Amyloid protein-binding protein 1, Proto-oncogene protein 1

UniProt: Q13564