ACTN1 antibody (AA 28-260)

Catalog No. ABIN7444730

The Rabbit Polyclonal anti-ACTN1 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect ACTN1 in samples from Human.

Quick Overview for ACTN1 antibody (AA 28-260) (ABIN7444730)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-ACTN1 Antibody

Binding Specificity: AA 28-260

Purpose: Polyclonal Antibody to Actinin Alpha 1 (ACTN1)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACTN1. It has been selected for its ability to recognize ACTN1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Actinin Alpha 1 (ACTN1) corresdonding to Ala28~Asn260 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background: Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

UniProt: P12814

Pathways: Cell-Cell Junction Organization