CUBN antibody (AA 3157-3274)

Catalog No. ABIN7434884

This Rabbit Polyclonal antibody specifically detects CUBN in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for CUBN antibody (AA 3157-3274) (ABIN7434884)

Target: CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CUBN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CUBN Antibody

Binding Specificity: AA 3157-3274

Purpose: Polyclonal Antibody to Cubilin (CUBN)

Specificity: The antibody is a rabbit polyclonal antibody raised against CUBN. It has been selected for its ability to recognize CUBN in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cubilin (CUBN) corresdonding to Cys3157~Met3274 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:420-1660Immunohistochemistry: 5-20 μg/mL,1:42-166Immunocytochemistry: 5-20 μg/mL,1:42-166Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.83 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CUBN

Target: CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Alternative Name: CUBN

Background: IFCR, MGA1, gp280, Intrinsic Factor Cobalamin Receptor, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-vitamin B12 receptor

UniProt: O60494

Pathways: Metabolism of Steroid Hormones and Vitamin D, Lipid Metabolism