KERA antibody (AA 21-292)

Catalog No. ABIN7444004

The Rabbit Polyclonal anti-KERA antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect KERA in samples from Cow.

Quick Overview for KERA antibody (AA 21-292) (ABIN7444004)

Target: KERA (Keratocan (KERA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KERA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-KERA Antibody

Binding Specificity: AA 21-292

Purpose: Polyclonal Antibody to Keratocan (KERA)

Specificity: The antibody is a rabbit polyclonal antibody raised against KERA. It has been selected for its ability to recognize KERA in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Keratocan (KERA) corresdonding to Arg21~Asn292

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for KERA

Target: KERA (Keratocan (KERA))

Alternative Name: KERA

Background: CNA2, SLRR2B, Keratocan Proteoglycan, Keratan sulfate proteoglycan keratocan

UniProt: O62702

Pathways: Glycosaminoglycan Metabolic Process