SFRP5 antibody (AA 45-162)

Catalog No. ABIN7435169

This anti-SFRP5 antibody is a Rabbit Polyclonal antibody detecting SFRP5 in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for SFRP5 antibody (AA 45-162) (ABIN7435169)

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFRP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SFRP5 Antibody

Binding Specificity: AA 45-162

Purpose: Polyclonal Antibody to Secreted Frizzled Related Protein 5 (SFRP5)

Specificity: The antibody is a rabbit polyclonal antibody raised against SFRP5. It has been selected for its ability to recognize SFRP5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Secreted Frizzled Related Protein 5 (SFRP5) corresdonding to Ser45~Val162 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SFRP5

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Alternative Name: SFRP5

Background: SARP3, Secreted Apoptosis Related Protein 3, Frizzled-related protein 1b

UniProt: D3ZTV6

Pathways: WNT Signaling