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Sclerostin antibody (AA 25-209)

This anti-Sclerostin antibody is a Rabbit Polyclonal antibody detecting Sclerostin in WB, IHC, IP and ICC. Suitable for Mouse.
Catalog No. ABIN7435193

Quick Overview for Sclerostin antibody (AA 25-209) (ABIN7435193)

Target

See all Sclerostin (SOST) Antibodies
Sclerostin (SOST)

Reactivity

  • 66
  • 53
  • 37
  • 16
  • 15
  • 15
  • 2
  • 1
  • 1
  • 1
  • 1
Mouse

Host

  • 68
  • 6
  • 2
  • 1
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Rabbit

Clonality

  • 69
  • 9
Polyclonal

Conjugate

  • 35
  • 6
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  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
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  • 1
  • 1
This Sclerostin antibody is un-conjugated

Application

  • 74
  • 32
  • 29
  • 24
  • 13
  • 11
  • 10
  • 9
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

    • 15
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    • 1
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    • 1
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    AA 25-209

    Purpose

    Polyclonal Antibody to Sclerostin (SOST)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against SOST. It has been selected for its ability to recognize SOST in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant Sclerostin (SOST) corresdonding to Gly25~Asn209 with N-terminal His and GST Tag

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.6 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Expiry Date

    24 months
  • Target

    Sclerostin (SOST)

    Alternative Name

    SOST

    Background

    VBCH, Sclerosteosis

    UniProt

    Q99P68
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