CYP24A1 antibody (AA 260-514)

Catalog No. ABIN7440206

The Rabbit Polyclonal anti-CYP24A1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CYP24A1 in samples from Mouse.

Quick Overview for CYP24A1 antibody (AA 260-514) (ABIN7440206)

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP24A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CYP24A1 Antibody

Binding Specificity: AA 260-514

Purpose: Polyclonal Antibody to Cytochrome P450 24A1 (CYP24A1)

Specificity: The antibody is a rabbit polyclonal antibody raised against CYP24A1. It has been selected for its ability to recognize CYP24A1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cytochrome P450 24A1 (CYP24A1) corresdonding to His260~Arg514 (Accession # Q64441)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:200-900,Immunohistochemistry: 5-20 μg/mL,1:20-90,Immunocytochemistry: 5-20 μg/mL,1:20-90,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CYP24A1

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Alternative Name: CYP24A1

Background: CP24, CYP24, P450-CC24, Vitamin D 24-Hydroxylase, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial , Cytochrome P450 Family 24 Subfamily A Polypeptide 1

UniProt: Q64441

Pathways: Metabolism of Steroid Hormones and Vitamin D