GCLC antibody (AA 435-636)

Catalog No. ABIN7435583

This Rabbit Polyclonal antibody specifically detects GCLC in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for GCLC antibody (AA 435-636) (ABIN7435583)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GCLC Antibody

Binding Specificity: AA 435-636

Purpose: Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)

Specificity: The antibody is a rabbit polyclonal antibody raised against GCLC. It has been selected for its ability to recognize GCLC in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) corresdonding to Asp435~Ser636 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:450-1800Immunohistochemistry: 5-20 μg/mL,1:45-180Immunocytochemistry: 5-20 μg/mL,1:45-180Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background: GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS

UniProt: P97494

Pathways: Cell RedoxHomeostasis