Asparagine Synthetase antibody (AA 213-536)

Catalog No. ABIN7435586

The Rabbit Polyclonal anti-Asparagine Synthetase antibody has been validated for WB, ELISA, ICC, IHC (p) and IHC (fro). It is suitable to detect Asparagine Synthetase in samples from Human.

Quick Overview for Asparagine Synthetase antibody (AA 213-536) (ABIN7435586)

Target: Asparagine Synthetase (ASNS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Asparagine Synthetase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Asparagine Synthetase Antibody

Binding Specificity: AA 213-536

Purpose: Polyclonal Antibody to Asparagine Synthetase (ASNS)

Specificity: The antibody is a rabbit polyclonal antibody raised against ASNS. It has been selected for its ability to recognize ASNS in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Asparagine Synthetase (ASNS) corresdonding to His213~Tyr536 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Asparagine Synthetase

Target: Asparagine Synthetase (ASNS)

Alternative Name: ASNS

Background: Aspartate-Ammonia Ligase, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

UniProt: P08243

Pathways: ER-Nucleus Signaling