MCM5 antibody (AA 331-537)

Catalog No. ABIN7435823

Product Details anti-MCM5 Antibody

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Binding Specificity: AA 331-537

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Minichromosome Maintenance Deficient 5 (MCM5)

Specificity: The antibody is a rabbit polyclonal antibody raised against MCM5. It has been selected for its ability to recognize MCM5 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Minichromosome Maintenance Deficient 5 (MCM5) corresdonding to Val331~Ile537 (Accession # P33992)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:250-1000,Immunohistochemistry: 5-20 μg/mL,1:25-100,Immunocytochemistry: 5-20 μg/mL,1:25-100,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5 (MCM5 Products)

Background: CDC46, P1-CDC46, Cell Division Cycle 46, DNA replication licensing factor MCM5

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA