SPON1 antibody (AA 608-802)

Catalog No. ABIN7440035

The Rabbit Polyclonal anti-SPON1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect SPON1 in samples from Mouse.

Quick Overview for SPON1 antibody (AA 608-802) (ABIN7440035)

Target: SPON1 (Spondin 1 (SPON1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPON1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SPON1 Antibody

Binding Specificity: AA 608-802

Purpose: Polyclonal Antibody to Spondin 1 (SPON1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SPON1. It has been selected for its ability to recognize SPON1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Spondin 1 (SPON1) corresdonding to Pro608~Cys802 (Accession # Q8VCC9)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000,Immunohistochemistry: 5-20 μg/mL,1:50-200,Immunocytochemistry: 5-20 μg/mL,1:50-200,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SPON1

Target: SPON1 (Spondin 1 (SPON1))

Alternative Name: SPON1

Background: VSGP, F-spondin, F-spondin,Extracellular Matrix Protein, Vascular smooth muscle cell growth-promoting factor

UniProt: Q8VCC9